Hotspot

rps16

LSC · 5118-6248 · 1131 bp

Back to Galinsoga

Pi 0.00177

MarkerSeek score 59.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1131
variable_sites: 2
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAATAGAGCAAGAAAAGAAGG	TCTCTGTACCGACTGAACCA	39.1	50.0	56.9	57.7	1317-1324 bp	1.0	44.9
rps16_p2	ACGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	37.5	50.0	58.2	57.7	1317-1324 bp	1.0	44.2
rps16_p3	CGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	39.1	50.0	56.6	57.7	1316-1323 bp	1.0	42.9
rps16_p4	ACGAATAGAGCAAGAAAAGAAGG	GTCTCTGTACCGACTGAACC	39.1	55.0	56.9	57.4	1318-1325 bp	1.0	42.1
rps16_p5	ACGAATAGAGCAAGAAAAGAAGGA	GTCTCTGTACCGACTGAACC	37.5	55.0	58.2	57.4	1318-1325 bp	1.0	41.4