Hotspot

ndhD

SSC · 119815-121317 · 1503 bp

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Pi 0.002

MarkerSeek score 48.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 3
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCACAGGTTTTTCTGGTCCA	TCATTAGCCAACCAACTCTT	50.0	40.0	58.6	54.5	1594 bp	1.0	47.7
ndhD_p2	GCACAGGTTTTTCTGGTCCA	TTCATTAGCCAACCAACTCT	50.0	40.0	58.6	54.5	1595 bp	1.0	47.7
ndhD_p3	GCACAGGTTTTTCTGGTCCA	TTCATTAGCCAACCAACTCTT	50.0	38.1	58.6	55.4	1595 bp	1.0	46.5
ndhD_p4	GCACAGGTTTTTCTGGTCCA	TTTCATTAGCCAACCAACTCT	50.0	38.1	58.6	55.4	1596 bp	1.0	46.5
ndhD_p5	TTGGGCACAGGTTTTTCTGG	TCATTAGCCAACCAACTCTT	50.0	40.0	58.9	54.5	1598 bp	1.0	45.5