Hotspot

rpl32-ndhF

SSC · 123624-124528 · 905 bp

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Pi 0.0011

MarkerSeek score 59.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 905
variable_sites: 1
pi: 0.0011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CGCCCCATATCCTTTCCTTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1084 bp	1.0	76.9
rpl32-ndhF_p2	CGCCCCATATCCTTTCCTTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1082 bp	1.0	76.8
rpl32-ndhF_p3	CGCCCCATATCCTTTCCTTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1083 bp	1.0	76.8
rpl32-ndhF_p4	CGCCCCATATCCTTTCCTTT	CTTTTTGTTGCCGTCGGAAA	50.0	45.0	57.6	57.8	1090 bp	1.0	75.6
rpl32-ndhF_p5	CGCCCCATATCCTTTCCTTT	TTTGTTGCCGTCGGAAAAAG	50.0	45.0	57.6	57.8	1087 bp	1.0	75.6