Hotspot

trnL-UAG-rpl32

SSC · 122722-123458 · 737 bp

Back to Galinsoga

Pi 0.00272
MarkerSeek score 57.40
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
737
variable_sites
2
pi
0.00272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAAGGAAAGGATATGGGGCG 50.0 50.0 58.0 57.6 870-890 bp 1.0 77.3
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAAGGAAAGGATATGGGGCG 50.0 50.0 58.0 57.6 871-891 bp 1.0 77.3
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA AAAGGAAAGGATATGGGGCG 50.0 50.0 57.9 57.6 880-900 bp 1.0 76.4
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC AAAGGAAAGGATATGGGGCG 55.0 50.0 57.8 57.6 879-899 bp 1.0 75.8
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC AAAGGAAAGGATATGGGGCG 55.0 50.0 57.8 57.6 878-898 bp 1.0 75.8