Hotspot

ycf1-rps15

SSC · 113124-113528 · 405 bp

Back to Galinsoga

Pi 0.00494

MarkerSeek score 57.50

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 405
variable_sites: 2
pi: 0.00494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	GGGAGCCGCTTTAGTATGTT	CAGATCCGCTGTTTTCTTTGT	50.0	42.9	57.7	57.1	517 bp	1.0	62.5
ycf1-rps15_p2	ATGGGAGCCGCTTTAGTATG	CAGATCCGCTGTTTTCTTTGT	50.0	42.9	57.4	57.1	519 bp	1.0	60.7
ycf1-rps15_p3	CCAATAATGGGAGCCGCTTT	CAGATCCGCTGTTTTCTTTGT	50.0	42.9	58.6	57.1	525 bp	1.0	60.3
ycf1-rps15_p4	GGGAGCCGCTTTAGTATGTT	CAGATCCGCTGTTTTCTTTGTT	50.0	40.9	57.7	57.8	517 bp	1.0	59.9
ycf1-rps15_p5	TGGGAGCCGCTTTAGTATGT	CAGATCCGCTGTTTTCTTTGT	50.0	42.9	58.8	57.1	518 bp	1.0	58.7