Hotspot

rps12-ycf15

IRb · 97591-98576 · 986 bp

Back to Galinsoga

Pi 0.00101

MarkerSeek score 59.30

Primer available yes

Label rps12-ycf15

Feature Metadata

label_name: rps12-ycf15
feature_type: igs
region: IRb
length_bp: 986
variable_sites: 1
pi: 0.00101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ycf15_p1	TGGCAACTTTACGTAAGGCA	CCTTGCTTCTCTCACGGTAC	45.0	55.0	57.7	58.0	1132 bp	1.0	78.0
rps12-ycf15_p2	TGGCAACTTTACGTAAGGCA	TGACCTCCTTGCTTCTCTCA	45.0	50.0	57.7	58.0	1138 bp	1.0	77.9
rps12-ycf15_p3	TGGCAACTTTACGTAAGGCA	TCCTTGCTTCTCTCACGGTA	45.0	50.0	57.7	58.1	1133 bp	1.0	77.4
rps12-ycf15_p4	TGGCAACTTTACGTAAGGCA	GGTTGACCTCCTTGCTTCTC	45.0	55.0	57.7	58.2	1141 bp	1.0	76.5
rps12-ycf15_p5	TGGCAACTTTACGTAAGGCA	CACGGTACAATCCTCTTCCC	45.0	55.0	57.7	57.7	1120 bp	1.0	75.5