Hotspot

rbcL-accD

LSC · 56134-56695 · 562 bp

Back to Galinsoga

Pi 0.00356

MarkerSeek score 60.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 2
pi: 0.00356
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGGCTACCAAATGGAGTCCT	CCAAGACTGCTCGTTGAGTT	50.0	50.0	58.0	58.1	767 bp	1.0	79.0
rbcL-accD_p2	GGCTACCAAATGGAGTCCTG	CCAAGACTGCTCGTTGAGTT	55.0	50.0	58.0	58.1	766 bp	1.0	78.8
rbcL-accD_p3	AGGCTACCAAATGGAGTCCT	ATAGGACCAAGACTGCTCGT	50.0	50.0	58.0	58.1	773 bp	1.0	78.8
rbcL-accD_p4	GGCTACCAAATGGAGTCCTG	ATAGGACCAAGACTGCTCGT	55.0	50.0	58.0	58.1	772 bp	1.0	78.7
rbcL-accD_p5	AGGCTACCAAATGGAGTCCT	TAGGACCAAGACTGCTCGTT	50.0	50.0	58.0	58.4	772 bp	1.0	77.0