Hotspot

trnK-UUU-rps16

LSC · 4343-5117 · 775 bp

Back to Galinsoga

Pi 0.00129
MarkerSeek score 59.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
775
variable_sites
1
pi
0.00129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 972-973 bp 1.0 76.9
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 971-972 bp 1.0 76.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 911-912 bp 1.0 76.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 910-911 bp 1.0 76.2
trnK-UUU-rps16_p5 TGGTATGGACGAATCCCTCA TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 968-969 bp 1.0 76.2