primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4343	5117	972-973	1.000000	76.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4343	5117	971-972	1.000000	76.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4343	5117	911-912	1.000000	76.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4343	5117	910-911	1.000000	76.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TGGTATGGACGAATCCCTCA	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.482104	4343	5117	968-969	1.000000	76.2
rps16_p1	rps16	1	ACGAATAGAGCAAGAAAAGAAGG	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.9	57.7	0.0	0.0	4.403459	5118	6248	1317-1324	1.000000	44.9
rps16_p2	rps16	2	ACGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	24	20	37.5	50.0	58.2	57.7	0.0	0.0	4.486645	5118	6248	1317-1324	1.000000	44.2
rps16_p3	rps16	3	CGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.6	57.7	0.0	0.0	4.654102	5118	6248	1316-1323	1.000000	42.9
rps16_p4	rps16	4	ACGAATAGAGCAAGAAAAGAAGG	GTCTCTGTACCGACTGAACC	23	20	39.1	55.0	56.9	57.4	0.0	0.0	4.755119	5118	6248	1318-1325	1.000000	42.1
rps16_p5	rps16	5	ACGAATAGAGCAAGAAAAGAAGGA	GTCTCTGTACCGACTGAACC	24	20	37.5	55.0	58.2	57.4	0.0	0.0	4.838305	5118	6248	1318-1325	1.000000	41.4
rps2_p1	rps2	1	CTGTGCATCTACGCCAATCT	TGAGATTCAGAAATAGTAACCGA	20	23	50.0	34.8	58.1	54.9	0.0	0.0	6.131959	23453	24163	899-899	1.000000	40.1
rps2_p2	rps2	2	TGCATCTACGCCAATCTACG	TGAGATTCAGAAATAGTAACCGA	20	23	50.0	34.8	57.9	54.9	0.0	0.0	6.211548	23453	24163	896-896	1.000000	40.1
rps2_p3	rps2	3	TTTTTCAAAAGGGGGAGGCA	TGAGATTCAGAAATAGTAACCGA	20	23	45.0	34.8	57.8	54.9	0.0	0.0	6.259863	23453	24163	821-821	1.000000	40.1
rps2_p4	rps2	4	TTTTCAAAAGGGGGAGGCAG	TGAGATTCAGAAATAGTAACCGA	20	23	50.0	34.8	58.3	54.9	0.0	0.0	6.361561	23453	24163	820-820	1.000000	40.1
rps2_p5	rps2	5	CTGTGCATCTACGCCAATCT	TTGAGATTCAGAAATAGTAACCGA	20	24	50.0	33.3	58.1	55.6	0.0	0.0	6.406229	23453	24163	900-900	1.000000	40.1
psbC_p1	psbC	1	TTTCTCAAGAAATTCGCGCG	GGGAACCGAAGTGACTCCTA	20	20	45.0	55.0	57.8	58.4	26.8	0.0	0.672556	33398	34819	1576-1576	1.000000	74.6
psbC_p2	psbC	2	CGGCGGAAGATCCTGAATTT	GGGAACCGAAGTGACTCCTA	20	20	50.0	55.0	58.3	58.4	0.0	0.0	0.777874	33398	34819	1558-1558	1.000000	73.8
psbC_p3	psbC	3	AGGTATTCGTGCTTGGATGG	GGGAACCGAAGTGACTCCTA	20	20	50.0	55.0	57.7	58.4	0.0	0.0	0.778357	33398	34819	1503-1503	1.000000	73.8
psbC_p4	psbC	4	GGCGGAAGATCCTGAATTTG	GGGAACCGAAGTGACTCCTA	20	20	50.0	55.0	56.9	58.4	0.0	0.0	1.589892	33398	34819	1557-1557	1.000000	67.3
psbC_p5	psbC	5	TATTCGTGCTTGGATGGCG	GGGAACCGAAGTGACTCCTA	19	20	52.6	55.0	58.3	58.4	0.0	0.0	1.757210	33398	34819	1500-1500	1.000000	65.9
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	TCTTCGTAGGGCATTTGTGG	GAGAGATGGCTGAGTGGTTG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.129305	34820	35053	429-429	1.000000	79.7
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.181932	34820	35053	346-346	1.000000	79.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TCTTCGTAGGGCATTTGTGG	GTCTTTCGGAGAGATGGCTG	20	20	50.0	55.0	57.9	58.1	0.0	1.2	0.174703	34820	35053	437-437	1.000000	79.3
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CCTGTTCTTTCCATGACCCC	GTCTTTCGGAGAGATGGCTG	20	20	55.0	55.0	58.2	58.1	0.0	1.2	0.227330	34820	35053	354-354	1.000000	79.1
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TCTTCGTAGGGCATTTGTGG	TCTTTCGGAGAGATGGCTGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.255317	34820	35053	436-436	1.000000	78.7
psaA-ycf3_p1	psaA-ycf3	1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.487535	41384	42106	915-918	1.000000	76.2
psaA-ycf3_p2	psaA-ycf3	2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.488764	41384	42106	917-920	1.000000	76.2
psaA-ycf3_p3	psaA-ycf3	3	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	20	20	55.0	45.0	57.8	57.5	18.5	0.0	0.716540	41384	42106	916-919	1.000000	74.4
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.745852	41384	42106	850-853	1.000000	74.1
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.747080	41384	42106	852-855	1.000000	74.1
atpB-rbcL_p1	atpB-rbcL	1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.047103	53893	54675	939-944	1.000000	72.0
atpB-rbcL_p2	atpB-rbcL	2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.080391	53893	54675	938-943	1.000000	71.8
atpB-rbcL_p3	atpB-rbcL	3	GGCAACCCCAGAACTAGAAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.132608	53893	54675	866-871	1.000000	71.4
atpB-rbcL_p4	atpB-rbcL	4	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.141542	53893	54675	896-901	1.000000	71.3
atpB-rbcL_p5	atpB-rbcL	5	GGCAACCCCAGAACTAGAAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	57.9	58.1	0.0	0.0	1.165895	53893	54675	865-870	1.000000	71.1
rbcL-accD_p1	rbcL-accD	1	AGGCTACCAAATGGAGTCCT	CCAAGACTGCTCGTTGAGTT	20	20	50.0	50.0	58.0	58.1	11.3	0.0	0.156332	56134	56695	767-767	1.000000	79.0
rbcL-accD_p2	rbcL-accD	2	GGCTACCAAATGGAGTCCTG	CCAAGACTGCTCGTTGAGTT	20	20	55.0	50.0	58.0	58.1	11.3	0.0	0.173043	56134	56695	766-766	1.000000	78.8
rbcL-accD_p3	rbcL-accD	3	AGGCTACCAAATGGAGTCCT	ATAGGACCAAGACTGCTCGT	20	20	50.0	50.0	58.0	58.1	11.3	0.0	0.173449	56134	56695	773-773	1.000000	78.8
rbcL-accD_p4	rbcL-accD	4	GGCTACCAAATGGAGTCCTG	ATAGGACCAAGACTGCTCGT	20	20	55.0	50.0	58.0	58.1	11.3	0.0	0.190160	56134	56695	772-772	1.000000	78.7
rbcL-accD_p5	rbcL-accD	5	AGGCTACCAAATGGAGTCCT	TAGGACCAAGACTGCTCGTT	20	20	50.0	50.0	58.0	58.4	11.3	0.0	0.398370	56134	56695	772-772	1.000000	77.0
accD-psaI_p1	accD-psaI	1	TCCAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	21	20	47.6	45.0	57.2	58.0	0.0	1.6	1.753724	58148	58864	898-898	1.000000	66.1
accD-psaI_p2	accD-psaI	2	CCAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	21	20	47.6	45.0	57.2	58.0	0.0	1.6	1.761009	58148	58864	897-897	1.000000	66.0
accD-psaI_p3	accD-psaI	3	TTCCAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	20	20	45.0	45.0	56.2	58.0	0.0	1.6	1.761018	58148	58864	899-899	1.000000	66.0
accD-psaI_p4	accD-psaI	4	TCCAGAAGGGCTTATTCGAT	AAAGAAGCCATTGCGATTGC	20	20	45.0	45.0	56.0	58.0	0.0	1.6	1.996346	58148	58864	898-898	1.000000	64.1
accD-psaI_p5	accD-psaI	5	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	21	20	47.6	45.0	57.2	58.3	0.0	8.0	2.008062	58148	58864	888-888	1.000000	64.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	64205	65481	1469-1473	1.000000	72.2
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	64205	65481	1392-1396	1.000000	71.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	64205	65481	1478-1482	1.000000	71.2
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.125378	64205	65481	1483-1487	1.000000	71.1
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	64205	65481	1477-1481	1.000000	71.1
clpP_p1	clpP	1	AAACTCGGCAGGGTTCATTT	GGCTTTGTTACGTTTCCACA	20	20	45.0	45.0	58.0	56.9	1.5	0.0	1.143994	69493	71507	2124-2131	1.000000	71.0
clpP_p2	clpP	2	AAACTCGGCAGGGTTCATTT	AGGCTTTGTTACGTTTCCACA	20	21	45.0	42.9	58.0	58.4	1.5	0.0	1.358822	69493	71507	2125-2132	1.000000	69.2
clpP_p3	clpP	3	AAACTCGGCAGGGTTCATTT	AGGCTTTGTTACGTTTCCAC	20	20	45.0	45.0	58.0	56.6	1.5	0.0	1.439445	69493	71507	2125-2132	1.000000	68.6
clpP_p4	clpP	4	AACTCGGCAGGGTTCATTTC	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	58.5	56.9	0.0	0.0	1.604642	69493	71507	2123-2130	1.000000	67.3
clpP_p5	clpP	5	CGGCAGGGTTCATTTCAGAA	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	58.5	56.9	0.0	0.0	1.605745	69493	71507	2119-2126	1.000000	67.3
rps12-ycf15_p1	rps12-ycf15	1	TGGCAACTTTACGTAAGGCA	CCTTGCTTCTCTCACGGTAC	20	20	45.0	55.0	57.7	58.0	16.5	0.0	0.260022	97591	98576	1132-1132	1.000000	78.0
rps12-ycf15_p2	rps12-ycf15	2	TGGCAACTTTACGTAAGGCA	TGACCTCCTTGCTTCTCTCA	20	20	45.0	50.0	57.7	58.0	16.5	0.0	0.273147	97591	98576	1138-1138	1.000000	77.9
rps12-ycf15_p3	rps12-ycf15	3	TGGCAACTTTACGTAAGGCA	TCCTTGCTTCTCTCACGGTA	20	20	45.0	50.0	57.7	58.1	16.5	0.0	0.338450	97591	98576	1133-1133	1.000000	77.4
rps12-ycf15_p4	rps12-ycf15	4	TGGCAACTTTACGTAAGGCA	GGTTGACCTCCTTGCTTCTC	20	20	45.0	55.0	57.7	58.2	16.5	0.0	0.442898	97591	98576	1141-1141	1.000000	76.5
rps12-ycf15_p5	rps12-ycf15	5	TGGCAACTTTACGTAAGGCA	CACGGTACAATCCTCTTCCC	20	20	45.0	55.0	57.7	57.7	16.5	0.0	0.568815	97591	98576	1120-1120	1.000000	75.5
ycf1_p1	ycf1	1	AATGCAAGGGATGGATCACC	GATTTCCTTGAGACGGTCCC	20	20	50.0	55.0	57.9	58.0	0.0	4.1	0.103276	109830	109949	242-242	1.000000	79.8
ycf1_p2	ycf1	2	AATGCAAGGGATGGATCACC	GGATTTCCTTGAGACGGTCC	20	20	50.0	55.0	57.9	58.0	0.0	4.1	0.103276	109830	109949	243-243	1.000000	79.8
ycf1_p3	ycf1	3	AATGCAAGGGATGGATCACC	GGGATTTCCTTGAGACGGTC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.103276	109830	109949	244-244	1.000000	79.8
ycf1_p4	ycf1	4	AATGCAAGGGATGGATCACC	GAGACGGTCCCCAAATAACC	20	20	50.0	55.0	57.9	58.0	0.0	4.1	0.111792	109830	109949	233-233	1.000000	79.8
ycf1_p5	ycf1	5	GGATCACCAGCTTCGAACAA	GATTTCCTTGAGACGGTCCC	20	20	50.0	55.0	58.2	58.0	0.0	4.1	0.217178	109830	109949	230-230	1.000000	79.0
ycf1-rps15_p1	ycf1-rps15	1	GGGAGCCGCTTTAGTATGTT	CAGATCCGCTGTTTTCTTTGT	20	21	50.0	42.9	57.7	57.1	0.0	0.0	2.231336	113124	113528	517-517	1.000000	62.5
ycf1-rps15_p2	ycf1-rps15	2	ATGGGAGCCGCTTTAGTATG	CAGATCCGCTGTTTTCTTTGT	20	21	50.0	42.9	57.4	57.1	0.0	0.0	2.454967	113124	113528	519-519	1.000000	60.7
ycf1-rps15_p3	ycf1-rps15	3	CCAATAATGGGAGCCGCTTT	CAGATCCGCTGTTTTCTTTGT	20	21	50.0	42.9	58.6	57.1	0.0	0.0	2.501349	113124	113528	525-525	1.000000	60.3
ycf1-rps15_p4	ycf1-rps15	4	GGGAGCCGCTTTAGTATGTT	CAGATCCGCTGTTTTCTTTGTT	20	22	50.0	40.9	57.7	57.8	0.0	0.0	2.556112	113124	113528	517-517	1.000000	59.9
ycf1-rps15_p5	ycf1-rps15	5	TGGGAGCCGCTTTAGTATGT	CAGATCCGCTGTTTTCTTTGT	20	21	50.0	42.9	58.8	57.1	0.0	0.0	2.703617	113124	113528	518-518	1.000000	58.7
psaC-ndhD_p1	psaC-ndhD	1	ACAGAGGATTGTGTCGGTTG	AGAAAATGAAGGAACCTGCA	20	20	50.0	40.0	57.8	55.1	0.0	3.8	3.031363	119699	119814	297-351	1.000000	56.5
psaC-ndhD_p2	psaC-ndhD	2	CAGAGGATTGTGTCGGTTGT	AGAAAATGAAGGAACCTGCA	20	20	50.0	40.0	57.8	55.1	0.0	3.8	3.031363	119699	119814	296-350	1.000000	56.5
psaC-ndhD_p3	psaC-ndhD	3	AAGAGATGTGAATCCGCCTG	AGAAAATGAAGGAACCTGCA	20	20	50.0	40.0	57.7	55.1	0.0	3.8	3.183886	119699	119814	276-330	1.000000	55.3
psaC-ndhD_p4	psaC-ndhD	4	CAGAGGATTGTGTCGGTTGT	AAGAAAATGAAGGAACCTGCA	20	21	50.0	38.1	57.8	56.0	0.0	3.8	3.210220	119699	119814	297-351	1.000000	55.1
psaC-ndhD_p5	psaC-ndhD	5	CAGAGGATTGTGTCGGTTGT	AGAAAATGAAGGAACCTGCAA	20	21	50.0	38.1	57.8	56.0	0.0	5.6	3.210220	119699	119814	296-350	1.000000	55.1
ndhD_p1	ndhD	1	GCACAGGTTTTTCTGGTCCA	TCATTAGCCAACCAACTCTT	20	20	50.0	40.0	58.6	54.5	0.0	0.0	4.055890	119815	121317	1594-1594	1.000000	47.7
ndhD_p2	ndhD	2	GCACAGGTTTTTCTGGTCCA	TTCATTAGCCAACCAACTCT	20	20	50.0	40.0	58.6	54.5	0.0	0.0	4.055890	119815	121317	1595-1595	1.000000	47.7
ndhD_p3	ndhD	3	GCACAGGTTTTTCTGGTCCA	TTCATTAGCCAACCAACTCTT	20	21	50.0	38.1	58.6	55.4	0.0	0.0	4.205205	119815	121317	1595-1595	1.000000	46.5
ndhD_p4	ndhD	4	GCACAGGTTTTTCTGGTCCA	TTTCATTAGCCAACCAACTCT	20	21	50.0	38.1	58.6	55.4	0.0	0.0	4.205205	119815	121317	1596-1596	1.000000	46.5
ndhD_p5	ndhD	5	TTGGGCACAGGTTTTTCTGG	TCATTAGCCAACCAACTCTT	20	20	50.0	40.0	58.9	54.5	0.0	0.0	4.335744	119815	121317	1598-1598	1.000000	45.5
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AAAGGAAAGGATATGGGGCG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.372803	122722	123458	870-890	1.000000	77.3
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	AAAGGAAAGGATATGGGGCG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.374173	122722	123458	871-891	1.000000	77.3
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	TCCTAAGAGCAGCGTGTCTA	AAAGGAAAGGATATGGGGCG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.489250	122722	123458	880-900	1.000000	76.4
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CCTAAGAGCAGCGTGTCTAC	AAAGGAAAGGATATGGGGCG	20	20	55.0	50.0	57.8	57.6	0.0	0.0	0.561267	122722	123458	879-899	1.000000	75.8
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	CTAAGAGCAGCGTGTCTACC	AAAGGAAAGGATATGGGGCG	20	20	55.0	50.0	57.8	57.6	0.0	0.0	0.561267	122722	123458	878-898	1.000000	75.8
rpl32-ndhF_p1	rpl32-ndhF	1	CGCCCCATATCCTTTCCTTT	GTTGCCGTCGGAAAAAGAAG	20	20	50.0	50.0	57.6	58.0	0.0	17.5	0.397386	123624	124528	1084-1084	1.000000	76.9
rpl32-ndhF_p2	rpl32-ndhF	2	CGCCCCATATCCTTTCCTTT	TGCCGTCGGAAAAAGAAGAA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	123624	124528	1082-1082	1.000000	76.8
rpl32-ndhF_p3	rpl32-ndhF	3	CGCCCCATATCCTTTCCTTT	TTGCCGTCGGAAAAAGAAGA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	123624	124528	1083-1083	1.000000	76.8
rpl32-ndhF_p4	rpl32-ndhF	4	CGCCCCATATCCTTTCCTTT	CTTTTTGTTGCCGTCGGAAA	20	20	50.0	45.0	57.6	57.8	0.0	17.5	0.561267	123624	124528	1090-1090	1.000000	75.6
rpl32-ndhF_p5	rpl32-ndhF	5	CGCCCCATATCCTTTCCTTT	TTTGTTGCCGTCGGAAAAAG	20	20	50.0	45.0	57.6	57.8	0.0	17.5	0.561267	123624	124528	1087-1087	1.000000	75.6