Hotspot

ycf1

SSC · 121707-127304 · 5598 bp

Back to Galeopsis

Pi 0.0101

MarkerSeek score 50.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5598
variable_sites: 56
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTCGTTTATTCTAAGCAATTTTC	AGAATCGGGATCTACGAATCT	29.2	42.9	53.4	55.8	170 bp	1.0	42.4
ycf1_p2	TCTTCGTTTATTCTAAGCAATTT	GAATCGGGATCTACGAATCT	26.1	45.0	52.8	54.2	170 bp	1.0	42.4
ycf1_p3	TCTTCGTTTATTCTAAGCAATTT	AGAATCGGGATCTACGAATCT	26.1	42.9	52.8	55.8	171 bp	1.0	42.3
ycf1_p4	TCTTCGTTTATTCTAAGCAATTTTC	AGAATCGGGATCTACGAATCT	28.0	42.9	54.7	55.8	171 bp	1.0	42.3
ycf1_p5	TCTTCGTTTATTCTAAGCAATTTT	AGAATCGGGATCTACGAATCT	25.0	42.9	53.6	55.8	171 bp	1.0	42.3