Hotspot

ndhA

SSC · 117578-119721 · 2144 bp

Back to Galeopsis

Pi 0.00653

MarkerSeek score 50.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2144
variable_sites: 14
pi: 0.00653
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTTTCTCTTGTTTGAGACAA	TGGGAGAAGTTGATCGTTGA	33.3	45.0	52.9	56.5	2214 bp	1.0	40.5
ndhA_p2	TTCTTTCTCTTGTTTGAGACA	TGGGAGAAGTTGATCGTTGA	33.3	45.0	52.9	56.5	2215 bp	1.0	40.5
ndhA_p3	TTCTTTCTCTTGTTTGAGACAA	TGGGAGAAGTTGATCGTTGA	31.8	45.0	53.8	56.5	2215 bp	1.0	40.5
ndhA_p4	TTTCTTTCTCTTGTTTGAGACA	TGGGAGAAGTTGATCGTTGA	31.8	45.0	53.8	56.5	2216 bp	1.0	40.5
ndhA_p5	TCTTTCTCTTGTTTGAGACAA	TGGGAGAAGTTGATCGTTGAA	33.3	42.9	52.9	57.2	2214 bp	1.0	40.5