Hotspot

trnN-GUU-trnR-ACG

IRa · 127702-128267 · 566 bp

Back to Galeopsis

Pi 0.00189
MarkerSeek score 63.50
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
566
variable_sites
1
pi
0.00189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 669-706 bp 1.0 82.4
trnN-GUU-trnR-ACG_p2 CCGTTGTTCCTCAGTAGCTC GAACAGATCCAGTGGAGACG 55.0 55.0 58.0 57.7 706-743 bp 1.0 81.8
trnN-GUU-trnR-ACG_p3 TGGGTTGAGAACGGGAATTG CAGAGGATTAGAGCACGTGG 50.0 55.0 58.1 57.8 710-747 bp 1.0 81.6
trnN-GUU-trnR-ACG_p4 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 620-657 bp 1.0 81.1
trnN-GUU-trnR-ACG_p5 TGGGTTGAGAACGGGAATTG GAACAGATCCAGTGGAGACG 50.0 55.0 58.1 57.7 747-784 bp 1.0 80.9