Hotspot

rps15-ycf1

SSC · 121278-121706 · 429 bp

Back to Galeopsis

Pi 0.0123

MarkerSeek score 52.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 429
variable_sites: 5
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	CAATAATGGCAGCCGTTTCG	50.0	50.0	58.0	58.2	662-685 bp	1.0	82.4
rps15-ycf1_p2	CAGCAGTCGTTGACGTTTTC	AGAGATGTATCCGCGATTGC	50.0	50.0	58.0	57.9	652 bp	0.5	59.4
rps15-ycf1_p3	AGTCGTTGACGTTTTCCCAA	AGAGATGTATCCGCGATTGC	45.0	50.0	58.0	57.9	648 bp	0.5	59.2
rps15-ycf1_p4	CCGAGCCATAACAAATGGGA	AGAGATGTATCCGCGATTGC	50.0	50.0	57.9	57.9	1189 bp	0.5	59.0
rps15-ycf1_p5	AACACCATGCATTGATGGGT	AGAGATGTATCCGCGATTGC	45.0	50.0	58.1	57.9	908 bp	0.5	59.0