Hotspot

ndhD-psaC

SSC · 114991-115085 · 95 bp

Back to Galeopsis

Pi 0.0842

MarkerSeek score 71.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 95
variable_sites: 8
pi: 0.0842
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTTGCCCACTGTACATTGCT	GCTAAGCAAATTGCCTCTGC	45.0	50.0	58.0	58.1	630 bp	1.0	80.6
ndhD-psaC_p2	TTTGCCCACTGTACATTGCT	AGGACTGCGTTGGTTGTAAG	45.0	50.0	58.0	58.1	599 bp	1.0	80.3
ndhD-psaC_p3	TTTGCCCACTGTACATTGCT	GGACTGCGTTGGTTGTAAGA	45.0	50.0	58.0	58.1	598 bp	1.0	80.3
ndhD-psaC_p4	TTTGCCCACTGTACATTGCT	GTGCCCCACGGATGTATTAG	45.0	55.0	58.0	58.1	676 bp	1.0	80.2
ndhD-psaC_p5	TTTGCCCACTGTACATTGCT	TGATACCTTGGGACGGATGT	45.0	50.0	58.0	58.1	653 bp	1.0	80.2