Hotspot

rps11-rpl36

LSC · 78298-78399 · 102 bp

Back to Galeopsis

Pi 0.0882

MarkerSeek score 86.00

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 102
variable_sites: 9
pi: 0.0882
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TGCACTTTTACGTGACCCAA	GGGGGCGCATTAGAGTAATT	45.0	50.0	58.0	57.7	224-239 bp	1.0	80.6
rps11-rpl36_p2	TGCACTTTTACGTGACCCAA	ATGTCGACTAATCCGCAGAC	45.0	50.0	58.0	57.5	244-259 bp	1.0	78.6
rps11-rpl36_p3	GTGCACTTTTACGTGACCCA	GGGGGCGCATTAGAGTAATT	50.0	50.0	58.7	57.7	225-240 bp	1.0	75.3
rps11-rpl36_p4	TGCACTTTTACGTGACCCAA	GGGCGCATTAGAGTAATTTGC	45.0	47.6	58.0	58.0	222-237 bp	1.0	74.8
rps11-rpl36_p5	TGCACTTTTACGTGACCCAA	GTCGACTAATCCGCAGACG	45.0	57.9	58.0	58.1	242-257 bp	1.0	73.4