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psbM-trnD-GUC

LSC · 30231-30753 · 523 bp

Back to Galeopsis

Pi 0.021

MarkerSeek score 65.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 11
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAGCAAAAAGGCGGTAGGAA	ACTTTCCCCGGGATTGTAGT	45.0	50.0	58.0	58.3	675 bp	1.0	78.5
psbM-trnD-GUC_p2	AAGCAAAAAGGCGGTAGGAA	TCCCCGGGATTGTAGTTCAA	45.0	50.0	58.0	58.3	671 bp	1.0	78.5
psbM-trnD-GUC_p3	AAGCAAAAAGGCGGTAGGAA	TTCCCCGGGATTGTAGTTCA	45.0	50.0	58.0	58.3	672 bp	1.0	78.5
psbM-trnD-GUC_p4	AAGCAAAAAGGCGGTAGGAA	CCCCTATTTACTTTCCCCGG	45.0	55.0	58.0	57.3	684 bp	1.0	76.0
psbM-trnD-GUC_p5	AAGCAAAAAGGCGGTAGGAA	CCCTATTTACTTTCCCCGGG	45.0	55.0	58.0	57.3	683 bp	1.0	76.0