Hotspot

ndhC-trnI-AAU

Genome · 114345-114760 · 416 bp

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Pi 0.0373
MarkerSeek score 79.50
Primer available yes
Label ndhC-trnI-AAU

Feature Metadata

label_name
ndhC-trnI-AAU
feature_type
igs
region
Genome
length_bp
416
variable_sites
15
pi
0.0373
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnI-AAU_p1 GCCCTTTTCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 561-568 bp 1.0 79.9
ndhC-trnI-AAU_p2 GCCCTTTTCTAATCGGAGCT GCACGTTGGGTCTTTCAAAC 50.0 50.0 57.7 58.2 624-631 bp 1.0 79.7
ndhC-trnI-AAU_p3 GCCCTTTTCTAATCGGAGCT AATGCACGTTGGGTCTTTCA 50.0 45.0 57.7 58.3 627-634 bp 1.0 78.5
ndhC-trnI-AAU_p4 GCCCTTTTCTAATCGGAGCT ATGCACGTTGGGTCTTTCAA 50.0 45.0 57.7 58.3 626-633 bp 1.0 78.5
ndhC-trnI-AAU_p5 GGCCCTTTTCTAATCGGAGC GCACGTTGGGTCTTTCAAAC 55.0 50.0 58.7 58.2 625-632 bp 1.0 76.9