Hotspot

trnL-UAA-trnF-GAA

Genome · 111770-111902 · 133 bp

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Pi 0.0902
MarkerSeek score 82.80
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
Genome
length_bp
133
variable_sites
12
pi
0.0902
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 223-226 bp 1.0 80.1
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC CAAATGAATTGCCGGGAACC 50.0 50.0 57.5 58.0 246-249 bp 1.0 80.0
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 50.0 50.0 57.5 57.5 220-223 bp 1.0 76.9
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC TTCAGTCCTCTGCTCTACCG 50.0 55.0 57.5 58.5 200-203 bp 1.0 76.7
trnL-UAA-trnF-GAA_p5 TTCAAGTCCCTCTATCCCCA CAAATGAATTGCCGGGAACC 50.0 50.0 57.0 58.0 235-238 bp 1.0 76.3