Hotspot

trnG-GCC-trnM-CAU

Genome · 100683-100738 · 56 bp

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Pi 0.107
MarkerSeek score 77.00
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
Genome
length_bp
56
variable_sites
6
pi
0.107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 GTTCGATTCCCGCTATCCG CTTGAGGTCACGGGTTCAAA 57.9 50.0 58.2 58.0 114-147 bp 1.0 73.9
trnG-GCC-trnM-CAU_p2 GGGTTCGATTCCCGCTATC CTTGAGGTCACGGGTTCAAA 57.9 50.0 57.8 58.0 116-149 bp 1.0 73.6
trnG-GCC-trnM-CAU_p3 GGTTCGATTCCCGCTATCC CTTGAGGTCACGGGTTCAAA 57.9 50.0 57.8 58.0 115-148 bp 1.0 73.6
trnG-GCC-trnM-CAU_p4 GTTCGATTCCCGCTATCCG TGAGGTCACGGGTTCAAATC 57.9 50.0 58.2 57.8 112-145 bp 1.0 72.8
trnG-GCC-trnM-CAU_p5 GGTTCGATTCCCGCTATCC TGAGGTCACGGGTTCAAATC 57.9 50.0 57.8 57.8 113-146 bp 1.0 72.6