Hotspot

psbC-trnS-UGA

Genome · 99345-99559 · 215 bp

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Pi 0.0846
MarkerSeek score 79.90
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
Genome
length_bp
215
variable_sites
17
pi
0.0846
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TCGAGATTCTGAGCCTGTTC GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.1 58.0 336-350 bp 1.0 79.5
psbC-trnS-UGA_p2 TCGAGATTCTGAGCCTGTTC GGTTGATAGCTCCGGTCTTG 50.0 55.0 57.1 58.1 321-335 bp 1.0 79.5
psbC-trnS-UGA_p3 CGAGATTCTGAGCCTGTTCT GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.0 58.0 335-349 bp 1.0 79.4
psbC-trnS-UGA_p4 TCGAGATTCTGAGCCTGTTC GCTGAGTGGTTGATAGCTCC 50.0 55.0 57.1 58.1 328-342 bp 1.0 79.4
psbC-trnS-UGA_p5 TCGAGATTCTGAGCCTGTTC GGCTGAGTGGTTGATAGCTC 50.0 55.0 57.1 58.1 329-343 bp 1.0 79.4