Hotspot

trnC-GCA-petN

Genome · 91513-92489 · 977 bp

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Pi 0.0582
MarkerSeek score 78.30
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
Genome
length_bp
977
variable_sites
56
pi
0.0582
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 CGATATGGCCGAGTGGTAAG TTAAAGCAGCCCAAGCAAGA 55.0 45.0 57.9 58.0 1080-1178 bp 1.0 84.4
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.1 58.0 1040-1138 bp 1.0 84.4
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 50.0 50.0 58.1 57.8 1034-1132 bp 1.0 83.0
trnC-GCA-petN_p4 CGATATGGCCGAGTGGTAAG CAGCCCAAGCAAGACTTACT 55.0 50.0 57.9 57.8 1074-1172 bp 1.0 82.9
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.4 58.0 1058-1156 bp 1.0 82.2