Hotspot

trnQ-UUG-psbK

Genome · 69616-70316 · 701 bp

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Pi 0.0541
MarkerSeek score 82.20
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
Genome
length_bp
701
variable_sites
35
pi
0.0541
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GACCAAAACCCGTTGCCTTA AGGCTTCAGGTAATTTCGCC 50.0 50.0 58.7 58.2 781-827 bp 1.0 80.1
trnQ-UUG-psbK_p2 CGAATAACGGGACCAAAACC AGGCTTCAGGTAATTTCGCC 50.0 50.0 57.1 58.2 791-837 bp 1.0 78.2
trnQ-UUG-psbK_p3 AAAACCCGTTGCCTTACCAC AGGCTTCAGGTAATTTCGCC 50.0 50.0 59.0 58.2 777-823 bp 1.0 77.8
trnQ-UUG-psbK_p4 ACCAAAACCCGTTGCCTTAC AGGCTTCAGGTAATTTCGCC 50.0 50.0 59.0 58.2 780-826 bp 1.0 77.8
trnQ-UUG-psbK_p5 CAAAACCCGTTGCCTTACCA AGGCTTCAGGTAATTTCGCC 50.0 50.0 59.0 58.2 778-824 bp 1.0 77.8