Hotspot

psaJ-trnP-UGG

Genome · 59084-59660 · 577 bp

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Pi 0.0396
MarkerSeek score 78.00
Primer available yes
Label psaJ-trnP-UGG

Feature Metadata

label_name
psaJ-trnP-UGG
feature_type
igs
region
Genome
length_bp
577
variable_sites
22
pi
0.0396
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-trnP-UGG_p1 AATGTCAATGCATCCGGGAA AAAACAAACGCGCTACCAAG 45.0 45.0 57.9 57.9 728-733 bp 1.0 82.7
psaJ-trnP-UGG_p2 AATGTCAATGCATCCGGGAA CAAAACAAACGCGCTACCAA 45.0 45.0 57.9 58.2 729-734 bp 1.0 82.2
psaJ-trnP-UGG_p3 AATGTCAATGCATCCGGGAA TTGTACCCAAAACAAACGCG 45.0 45.0 57.9 57.8 736-741 bp 1.0 82.0
psaJ-trnP-UGG_p4 GAATGTCAATGCATCCGGGA AAAACAAACGCGCTACCAAG 50.0 45.0 58.3 57.9 729-734 bp 1.0 81.2
psaJ-trnP-UGG_p5 ATGTCAATGCATCCGGGAAT AAAACAAACGCGCTACCAAG 45.0 45.0 57.6 57.9 727-732 bp 1.0 80.8