Hotspot

clpP-rps12

Genome · 56164-56239 · 76 bp

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Pi 0.183
MarkerSeek score 76.90
Primer available yes
Label clpP-rps12

Feature Metadata

label_name
clpP-rps12
feature_type
igs
region
Genome
length_bp
76
variable_sites
13
pi
0.183
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-rps12_p1 GCTTCGTGGCAGATATCCAA CTCTAACCTTCCCAACCACG 50.0 55.0 58.0 57.9 843 bp 0.5 58.9
clpP-rps12_p2 GCTTCGTGGCAGATATCCAA GATTCGGTTTGCACCAATCG 50.0 50.0 58.0 58.1 374 bp 0.5 58.8
clpP-rps12_p3 GCTTCGTGGCAGATATCCAA CTAGTACATGTTCCTCGGCG 50.0 55.0 58.0 57.9 279 bp 0.5 58.7
clpP-rps12_p4 GCTTCGTGGCAGATATCCAA AGTCGCACATACACCCTAGT 50.0 50.0 58.0 58.2 294 bp 0.5 58.4
clpP-rps12_p5 GCTTCGTGGCAGATATCCAA AGTTCGGATTGGCTGTCTTG 50.0 50.0 58.0 58.2 223 bp 0.5 58.1