Hotspot

trnM-CAU-rpl23

Genome · 39310-40035 · 726 bp

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Pi 0.0924
MarkerSeek score 90.80
Primer available yes
Label trnM-CAU-rpl23

Feature Metadata

label_name
trnM-CAU-rpl23
feature_type
igs
region
Genome
length_bp
726
variable_sites
62
pi
0.0924
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
90.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-rpl23_p1 ATTGGCGAAGTCGTAGGTTC CGACCCAACGTTTTATTTCTGT 50.0 40.9 58.0 57.5 865-881 bp 1.0 69.8
trnM-CAU-rpl23_p2 GGCGAAGTCGTAGGTTCAAT CGACCCAACGTTTTATTTCTGT 50.0 40.9 58.0 57.5 862-878 bp 1.0 69.8
trnM-CAU-rpl23_p3 GAATGGTTAAAGCGCCCAAC CGACCCAACGTTTTATTTCTGT 50.0 40.9 58.0 57.5 890-906 bp 1.0 69.6
trnM-CAU-rpl23_p4 GGTAGTAGGATCCCCACGAA CGACCCAACGTTTTATTTCTGT 55.0 40.9 57.9 57.5 922-938 bp 1.0 68.9
trnM-CAU-rpl23_p5 GGCGAAGTCGTAGGTTCAAT TCGACCCAACGTTTTATTTCTG 50.0 40.9 58.0 57.3 863-879 bp 1.0 67.8