Hotspot

trnL-CAA-ycf2

Genome · 32646-33166 · 521 bp

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Pi 0.0465
MarkerSeek score 78.80
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
Genome
length_bp
521
variable_sites
23
pi
0.0465
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 GGTGGTGGAATGGTAGACAC CCGGACGAGATGGTTGTTAC 55.0 55.0 57.9 58.4 628-653 bp 1.0 82.3
trnL-CAA-ycf2_p2 CCTTGGTGGTGGAATGGTAG CCGGACGAGATGGTTGTTAC 55.0 55.0 57.9 58.4 632-657 bp 1.0 82.1
trnL-CAA-ycf2_p3 GGTGGTGGAATGGTAGACAC AAAGATGGCTTTTCCCGGAC 55.0 50.0 57.9 58.5 642-667 bp 1.0 81.5
trnL-CAA-ycf2_p4 CCTTGGTGGTGGAATGGTAG AAAGATGGCTTTTCCCGGAC 55.0 50.0 57.9 58.5 646-671 bp 1.0 81.3
trnL-CAA-ycf2_p5 TGGTGGTGGAATGGTAGACA CCGGACGAGATGGTTGTTAC 50.0 55.0 58.3 58.4 629-654 bp 1.0 81.1