Hotspot

trnE-UUC-rrn16

Genome · 25344-25649 · 306 bp

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Pi 0.14
MarkerSeek score 77.90
Primer available yes
Label trnE-UUC-rrn16

Feature Metadata

label_name
trnE-UUC-rrn16
feature_type
igs
region
Genome
length_bp
306
variable_sites
41
pi
0.14
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rrn16_p1 TGAATCGCCCCATCTATCCT CCGCGAATCCTCTTGAAAGA 50.0 50.0 58.0 58.0 1051-1064 bp 1.0 84.2
trnE-UUC-rrn16_p2 ATTGTACTGCGCTCTCCAAG CCTGTCCTCAGTTCGGATTG 50.0 55.0 58.0 58.0 1260-1268 bp 1.0 83.9
trnE-UUC-rrn16_p3 TGCTTGTTCCACCTTCTTCC CCTGTCCTCAGTTCGGATTG 50.0 55.0 58.0 58.0 1238-1246 bp 1.0 83.9
trnE-UUC-rrn16_p4 ATTGTACTGCGCTCTCCAAG CCGCGAATCCTCTTGAAAGA 50.0 50.0 58.0 58.0 1550-1558 bp 1.0 83.2
trnE-UUC-rrn16_p5 TGCTTGTTCCACCTTCTTCC CCGCGAATCCTCTTGAAAGA 50.0 50.0 58.0 58.0 1528-1536 bp 1.0 83.2