Hotspot

ycf1-trnN-GUU

Genome · 18574-19034 · 461 bp

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Pi 0.106
MarkerSeek score 82.90
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
Genome
length_bp
461
variable_sites
48
pi
0.106
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 TCAGGCATAACACTAGTAGA ACCACTGAGCTACTGAGGAA 40.0 50.0 52.5 57.7 530-708 bp 1.0 47.5
ycf1-trnN-GUU_p2 TTCAGGCATAACACTAGTAGA ACCACTGAGCTACTGAGGAA 38.1 50.0 53.4 57.7 531-709 bp 1.0 47.5
ycf1-trnN-GUU_p3 TTTCAGGCATAACACTAGTAGA ACCACTGAGCTACTGAGGAA 36.4 50.0 54.3 57.7 532-710 bp 1.0 47.5
ycf1-trnN-GUU_p4 TCAGGCATAACACTAGTAGAT ACCACTGAGCTACTGAGGAA 38.1 50.0 53.2 57.7 530-708 bp 1.0 47.5
ycf1-trnN-GUU_p5 GCTGAATTAACTATTTTCAGGCA ACCACTGAGCTACTGAGGAA 34.8 50.0 55.6 57.7 546-724 bp 1.0 47.3