Hotspot

ycf60

Genome · 115645-116583 · 939 bp

Back to Galdieria

Pi 0.242

MarkerSeek score 81.40

Primer available yes

Label ycf60

Feature Metadata

label_name: ycf60
feature_type: gene
region: Genome
length_bp: 939
variable_sites: 221
pi: 0.242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf60_p1	TTGCAAGACGTTTGGATGGT	GGGGAAGCTCTTTGGGAAAA	45.0	50.0	58.3	58.0	2062 bp	0.5	57.4
ycf60_p2	TTGCAAGACGTTTGGATGGT	ACACCTTGGTGCTTATAGCG	45.0	50.0	58.3	58.0	2148 bp	0.5	57.3
ycf60_p3	TTGCAAGACGTTTGGATGGT	CACCTTGGTGCTTATAGCGT	45.0	50.0	58.3	58.0	2147 bp	0.5	57.3
ycf60_p4	TTGCAAGACGTTTGGATGGT	GAAGCCCACCTCAAGATGAG	45.0	55.0	58.3	58.0	2362 bp	0.5	57.2
ycf60_p5	TTGCAAGACGTTTGGATGGT	TTTCGAACTCGGCTGTGAAA	45.0	45.0	58.3	58.1	2106 bp	0.5	57.0