Hotspot

ycf23

Genome · 104691-105494 · 804 bp

Back to Galdieria

Pi 0.246

MarkerSeek score 83.50

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: Genome
length_bp: 804
variable_sites: 188
pi: 0.246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	AGCGTGTCCTTGTGATGTTT	TAGGAGTTTTCCCTCGCTCA	45.0	50.0	58.0	58.1	1597 bp	0.5	59.2
ycf23_p2	AGCGTGTCCTTGTGATGTTT	CCAAGCACCAGCAGTATCAA	45.0	50.0	58.0	58.2	1725 bp	0.5	58.3
ycf23_p3	TGTAAGAGCGTGTCCTTGTG	TAGGAGTTTTCCCTCGCTCA	50.0	50.0	57.9	58.1	1603 bp	0.5	58.3
ycf23_p4	TGTGTAAGAGCGTGTCCTTG	TAGGAGTTTTCCCTCGCTCA	50.0	50.0	57.9	58.1	1605 bp	0.5	58.3
ycf23_p5	GTGTAAGAGCGTGTCCTTGT	TAGGAGTTTTCCCTCGCTCA	50.0	50.0	57.8	58.1	1604 bp	0.5	58.3