Hotspot

thiG

Genome · 113349-114185 · 837 bp

Back to Galdieria

Pi 0.197

MarkerSeek score 79.80

Primer available yes

Label thiG

Feature Metadata

label_name: thiG
feature_type: gene
region: Genome
length_bp: 837
variable_sites: 163
pi: 0.197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiG_p1	TCCAAGGCAAATCCCCAAAA	CCTACACCTCCTTTTCCGGA	45.0	55.0	57.9	58.7	1246 bp	0.5	53.3
thiG_p2	GTCTACCTGGACCTGGTGAT	CCTACACCTCCTTTTCCGGA	55.0	55.0	58.1	58.7	1169 bp	0.5	53.1
thiG_p3	TGATGTCCAAGGCAAATCCC	CCTACACCTCCTTTTCCGGA	50.0	55.0	58.1	58.7	1251 bp	0.5	53.0
thiG_p4	TCCAAGGCAAATCCCCAAAA	ACACCTCCTTTTCCGGAAGT	45.0	50.0	57.9	58.9	1243 bp	0.5	52.2
thiG_p5	GTCTACCTGGACCTGGTGAT	ACACCTCCTTTTCCGGAAGT	55.0	50.0	58.1	58.9	1166 bp	0.5	52.0