Hotspot

cysT

Genome · 89424-90239 · 816 bp

Back to Galdieria

Pi 0.229

MarkerSeek score 80.20

Primer available yes

Label cysT

Feature Metadata

label_name: cysT
feature_type: gene
region: Genome
length_bp: 816
variable_sites: 184
pi: 0.229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cysT_p1	AGACTGCACCAAATTCACCA	CCACTCGAAAAATCGCATGG	45.0	50.0	57.6	57.8	1997 bp	0.5	55.7
cysT_p2	AGACTGCACCAAATTCACCA	AAAATCGCATGGAGTGTTGC	45.0	45.0	57.6	57.6	1989 bp	0.5	54.3
cysT_p3	AACTTGCCAAGAGTTTGCAC	CCACTCGAAAAATCGCATGG	45.0	50.0	57.4	57.8	1903 bp	0.5	53.9
cysT_p4	AGACTGCACCAAATTCACCA	ATGGAGTGTTGCCAATGGAC	45.0	50.0	57.6	58.5	1981 bp	0.5	53.5
cysT_p5	AGACTGCACCAAATTCACCA	CATGGAGTGTTGCCAATGGA	45.0	50.0	57.6	58.5	1982 bp	0.5	53.5