Hotspot

cobA

Genome · 86597-87379 · 783 bp

Back to Galdieria

Pi 0.217

MarkerSeek score 79.90

Primer available yes

Label cobA

Feature Metadata

label_name: cobA
feature_type: gene
region: Genome
length_bp: 783
variable_sites: 168
pi: 0.217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cobA_p1	AGCCACCAAGACTCCAATTG	TGCGGGTTCTTTTGGAATCA	50.0	45.0	58.1	58.0	1396 bp	0.5	59.2
cobA_p2	AGCCACCAAGACTCCAATTG	TCACTTATGGCCATCAGTGC	50.0	50.0	58.1	58.0	1684 bp	0.5	59.0
cobA_p3	AGCCACCAAGACTCCAATTG	TGTTGGTTTTGTCCTGCCTT	50.0	45.0	58.1	58.1	1848 bp	0.5	58.2
cobA_p4	AGCCACCAAGACTCCAATTG	TTGTTGGTTTTGTCCTGCCT	50.0	45.0	58.1	58.1	1849 bp	0.5	58.2
cobA_p5	AGTTAGCCACCAAGACTCCA	TGCGGGTTCTTTTGGAATCA	50.0	45.0	58.3	58.0	1400 bp	0.5	57.7