Hotspot

ccsA

Genome · 106902-107813 · 912 bp

Back to Galdieria

Pi 0.169

MarkerSeek score 81.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 912
variable_sites: 150
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ATAGGAGAAGGGGGTTCTGG	ACACGTTGATTTTGCGTTGA	55.0	40.0	57.8	57.2	2048-2052 bp	1.0	83.4
ccsA_p2	ATAGGAGAAGGGGGTTCTGG	TCAACACGTTGATTTTGCGT	55.0	40.0	57.8	57.2	2051 bp	0.5	51.7
ccsA_p3	ATAGGAGAAGGGGGTTCTGG	GCGGTAAGCTGCCCAAATTA	55.0	50.0	57.8	58.9	2154 bp	0.5	51.2
ccsA_p4	ATAGGAGAAGGGGGTTCTGG	CACGTTGATTTTGCGTTGAGA	55.0	42.9	57.8	58.0	2047 bp	0.5	50.3
ccsA_p5	TTCTCCAGAAGCTTGCTCAG	ACACGTTGATTTTGCGTTGA	50.0	40.0	57.5	57.2	1962 bp	0.5	49.6