Hotspot

carA

Genome · 84007-85206 · 1200 bp

Back to Galdieria

Pi 0.245

MarkerSeek score 79.20

Primer available yes

Label carA

Feature Metadata

label_name: carA
feature_type: gene
region: Genome
length_bp: 1200
variable_sites: 293
pi: 0.245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
carA_p1	ACGAGATTCAAACTCAAAACGC	AGCTCCTATAGGTCATTTGCC	40.9	47.6	58.1	57.2	1549 bp	0.5	28.8
carA_p2	ACGAGATTCAAACTCAAAACGC	GCTCCTATAGGTCATTTGCCT	40.9	47.6	58.1	57.2	1548 bp	0.5	28.8
carA_p3	CGAGATTCAAACTCAAAACGCT	AGCTCCTATAGGTCATTTGCC	40.9	47.6	57.9	57.2	1548 bp	0.5	28.7
carA_p4	CGAGATTCAAACTCAAAACGCT	GCTCCTATAGGTCATTTGCCT	40.9	47.6	57.9	57.2	1547 bp	0.5	28.7
carA_p5	ACGAGATTCAAACTCAAAACGC	GCTCCTATAGGTCATTTGCCTT	40.9	45.5	58.1	57.9	1548 bp	0.5	26.3