Hotspot

accB

Genome · 6292-6843 · 552 bp

Back to Galdieria

Pi 0.14

MarkerSeek score 79.40

Primer available yes

Label accB

Feature Metadata

label_name: accB
feature_type: gene
region: Genome
length_bp: 552
variable_sites: 76
pi: 0.14
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accB_p1	TCTTACCGACACCTGGTCTT	AATCGTGAGCATCTGCATGT	50.0	45.0	58.0	58.0	1606 bp	0.5	59.7
accB_p2	TCTTACCGACACCTGGTCTT	AATCCTCCACCAACATCAGC	50.0	50.0	58.0	57.9	1835 bp	0.5	58.9
accB_p3	TCTTACCGACACCTGGTCTT	CCTCCACCAACATCAGCATT	50.0	50.0	58.0	58.2	1832 bp	0.5	58.7
accB_p4	TCTTACCGACACCTGGTCTT	TGATTCCTGAGGCTCTCCAT	50.0	50.0	58.0	57.8	1891 bp	0.5	58.5
accB_p5	TCTTACCGACACCTGGTCTT	CGTGAGCATCTGCATGTAGA	50.0	50.0	58.0	57.8	1603 bp	0.5	58.2