Hotspot

accA

Genome · 105533-106564 · 1032 bp

Back to Galdieria

Pi 0.176

MarkerSeek score 79.70

Primer available yes

Label accA

Feature Metadata

label_name: accA
feature_type: gene
region: Genome
length_bp: 1032
variable_sites: 180
pi: 0.176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accA_p1	TGCCCACTTTAATTGCCACT	GGGCTCATATTGGAGTTGGG	45.0	55.0	58.0	58.0	1884 bp	0.5	59.8
accA_p2	TGCCCACTTTAATTGCCACT	GGGGCTCATATTGGAGTTGG	45.0	55.0	58.0	58.0	1885 bp	0.5	59.8
accA_p3	TGCCCACTTTAATTGCCACT	GGAGCTGTATGGGCAAATGA	45.0	50.0	58.0	57.9	1910 bp	0.5	59.5
accA_p4	TGCCCACTTTAATTGCCACT	AGCTGTATGGGCAAATGAGG	45.0	50.0	58.0	57.9	1908 bp	0.5	59.4
accA_p5	TGCCCACTTTAATTGCCACT	AGGAGCTGTATGGGCAAATG	45.0	50.0	58.0	57.9	1911 bp	0.5	59.4