Hotspot

ndhF

SSC · 112977-115178 · 2202 bp

Back to Galanthus

Pi 0.00651

MarkerSeek score 46.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2202
variable_sites: 26
pi: 0.00651
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTTGAACGGACCTCATCGAG	TCCAATCAATCAGTTCCGCA	50.0	45.0	57.6	57.5	2699-2849 bp	1.0	78.3
ndhF_p2	TTTTGAACGGACCTCATCGA	TCCAATCAATCAGTTCCGCA	45.0	45.0	57.2	57.5	2700-2850 bp	1.0	74.6
ndhF_p3	TTTGAACGGACCTCATCGAG	ATCCAATCAATCAGTTCCGCA	50.0	42.9	57.6	58.0	2700-2850 bp	1.0	74.2
ndhF_p4	TTTGAACGGACCTCATCGAG	TCAATCAGTTCCGCAATGAGT	50.0	42.9	57.6	57.9	2694-2844 bp	1.0	73.7
ndhF_p5	TTTGAACGGACCTCATCGAG	TCCAATCAATCAGTTCCGCAA	50.0	42.9	57.6	58.2	2699-2849 bp	1.0	72.5