Hotspot

petD-rpoA

LSC · 79610-79762 · 153 bp

Back to Galanthus

Pi 0.0276

MarkerSeek score 60.10

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 6
pi: 0.0276
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CTCGAGCCGGATGATGAAAA	CTTTTCTACACGCGGAGGAA	50.0	50.0	58.1	57.9	1081-1087 bp	1.0	80.7
petD-rpoA_p2	CTCGAGCCGGATGATGAAAA	TTTTCTACACGCGGAGGAAG	50.0	50.0	58.1	57.9	1080-1086 bp	1.0	80.7
petD-rpoA_p3	CTCGAGCCGGATGATGAAAA	ATCCATTTGCGTTAGGGGTC	50.0	50.0	58.1	57.9	1446-1452 bp	1.0	80.6
petD-rpoA_p4	CTCGAGCCGGATGATGAAAA	TTTGCGTTAGGGGTCCTAGA	50.0	50.0	58.1	58.1	1441-1447 bp	1.0	80.5
petD-rpoA_p5	CTCGAGCCGGATGATGAAAA	TAGCTAACCTGACGGAACCA	50.0	50.0	58.1	58.1	1357-1363 bp	1.0	80.5