Hotspot

petA-psbJ

LSC · 65018-66151 · 1134 bp

Back to Galanthus

Pi 0.00999

MarkerSeek score 66.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 21
pi: 0.00999
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.7	58.0	1265-1270 bp	1.0	76.1
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTCT	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.7	58.1	1266-1271 bp	1.0	75.4
petA-psbJ_p3	GATCCATTACGCGTCCAAGG	CCGGATTGGGTTCATCTCTG	55.0	55.0	58.8	58.0	1276-1281 bp	1.0	75.2
petA-psbJ_p4	GATCCATTACGCGTCCAAGG	TCCGGATTGGGTTCATCTCT	55.0	50.0	58.8	58.1	1277-1282 bp	1.0	74.6
petA-psbJ_p5	TCCAAGGCCTTTTGTTCTTCT	CCGGATTGGGTTCATCTCTG	42.9	55.0	57.7	58.0	1263-1268 bp	1.0	71.0