Hotspot

trnL-UAA-trnF-GAA

LSC · 49290-49653 · 364 bp

Back to Galanthus

Pi 0.0156

MarkerSeek score 59.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 10
pi: 0.0156
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AGAGAGAGTCCCATTCTGCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.8	58.1	530-531 bp	1.0	79.1
trnL-UAA-trnF-GAA_p2	AGAGAGAGTCCCATTCTGCA	ATTCACGTGTCAGGAACCAG	50.0	50.0	57.8	57.8	551-552 bp	1.0	78.1
trnL-UAA-trnF-GAA_p3	AGAGAGAGTCCCATTCTGCA	CACGTGTCAGGAACCAGATT	50.0	50.0	57.8	57.8	548-549 bp	1.0	78.1
trnL-UAA-trnF-GAA_p4	GAGTCCCATTCTGCATGTCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.6	58.1	525-526 bp	1.0	77.8
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	454-455 bp	1.0	77.5