Hotspot

trnT-UGU-trnL-UAA

LSC · 47720-48692 · 973 bp

Back to Galanthus

Pi 0.00993

MarkerSeek score 49.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 15
pi: 0.00993
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCCATTTC	55.0	55.0	57.9	58.2	927-1035 bp	1.0	83.0
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	939-1047 bp	1.0	82.3
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCCATTTC	50.0	55.0	57.8	58.2	959-1067 bp	1.0	82.1
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	55.0	50.0	57.9	57.7	936-1044 bp	1.0	81.9
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	50.0	50.0	57.8	57.8	971-1079 bp	1.0	81.4