Hotspot

psaA-ycf3

LSC · 43124-43768 · 645 bp

Back to Galanthus

Pi 0.00726

MarkerSeek score 64.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 645
variable_sites: 9
pi: 0.00726
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	TGGTTGAAGATAACGAGGCG	50.0	50.0	57.9	58.0	752-754 bp	1.0	80.5
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	GGTTGAAGATAACGAGGCGT	50.0	50.0	57.9	58.0	751-753 bp	1.0	80.5
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	CGACCAAGCTGCTGAGTATT	50.0	50.0	57.9	58.0	822-824 bp	1.0	80.3
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	50.0	50.0	57.9	57.9	820-822 bp	1.0	79.5
psaA-ycf3_p5	GGCCCTTAGCTATTGTTCGT	TGGTTGAAGATAACGAGGCG	50.0	50.0	57.7	58.0	791-793 bp	1.0	78.6