Hotspot

psbC-trnS-UGA

LSC · 36679-36805 · 127 bp

Back to Galanthus

Pi 0.0381

MarkerSeek score 71.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 7
pi: 0.0381
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTGGCTGAGTGGTTGATAG	55.0	55.0	58.2	58.0	235-240 bp	1.0	82.5
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	224-229 bp	1.0	82.5
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GCTGAGTGGTTGATAGCTCC	55.0	55.0	58.2	58.1	231-236 bp	1.0	82.4
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GGCTGAGTGGTTGATAGCTC	55.0	55.0	58.2	58.1	232-237 bp	1.0	82.4
psbC-trnS-UGA_p5	TTCCATGACCCCTCTTAGCT	GGTGGCTGAGTGGTTGATAG	50.0	55.0	57.7	58.0	227-232 bp	1.0	81.8