Hotspot

rpoB-trnC-GCA

LSC · 27523-28749 · 1227 bp

Back to Galanthus

Pi 0.00844

MarkerSeek score 66.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1227
variable_sites: 18
pi: 0.00844
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCGGGTATTGTGGACATTCC	TAAAGGATTTGCAGTCCCCC	55.0	50.0	58.3	57.5	1294-1312 bp	1.0	75.9
rpoB-trnC-GCA_p2	CGGGTATTGTGGACATTCCC	TAAAGGATTTGCAGTCCCCC	55.0	50.0	58.3	57.5	1293-1311 bp	1.0	75.9
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1277-1295 bp	1.0	75.3
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1278-1296 bp	1.0	75.0
rpoB-trnC-GCA_p5	GCCAAGCCCTGATCAATGAA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	57.5	1348-1366 bp	1.0	74.2