Hotspot

rps2-rpoC2

LSC · 16979-17143 · 165 bp

Back to Galanthus

Pi 0.0295

MarkerSeek score 59.70

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 9
pi: 0.0295
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GAGCCCTTATTGCAGCTGAT	GAAGGTTGGAACGAGCGTAT	50.0	50.0	58.0	58.0	1099-1104 bp	1.0	80.9
rps2-rpoC2_p2	GAGCCCTTATTGCAGCTGAT	TTATCGATCCCAGGGGGTAC	50.0	55.0	58.0	58.0	983-988 bp	1.0	80.9
rps2-rpoC2_p3	AACATAATGACACCGAGCCC	GAAGGTTGGAACGAGCGTAT	50.0	50.0	58.0	58.0	1113-1118 bp	1.0	80.8
rps2-rpoC2_p4	GACATACCACCGAGCCATTT	GAAGGTTGGAACGAGCGTAT	50.0	50.0	58.0	58.0	1139-1144 bp	1.0	80.8
rps2-rpoC2_p5	GCGAGATCTGGGTCACAATT	GAAGGTTGGAACGAGCGTAT	50.0	50.0	58.0	58.0	1424-1429 bp	1.0	80.6