Hotspot

trnS-GCU-trnG-GCC

LSC · 8636-9950 · 1315 bp

Back to Galanthus

Pi 0.0111
MarkerSeek score 60.80
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1315
variable_sites
22
pi
0.0111
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.90
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CTCGTCCAACGGATTAGCAA CGAACGAATCACACTTTTACCA 50.0 40.9 58.0 57.3 1253-1428 bp 1.0 63.5
trnS-GCU-trnG-GCC_p2 AACTCGTCCAACGGATTAGC CGAACGAATCACACTTTTACCA 50.0 40.9 58.0 57.3 1255-1430 bp 1.0 63.5
trnS-GCU-trnG-GCC_p3 ATTAGCAATCCGCCGCTTTA CGAACGAATCACACTTTTACCA 45.0 40.9 58.0 57.3 1241-1416 bp 1.0 63.3
trnS-GCU-trnG-GCC_p4 CTCGTCCAACGGATTAGCAA TCGAACGAATCACACTTTTACC 50.0 40.9 58.0 57.0 1254-1429 bp 1.0 61.5
trnS-GCU-trnG-GCC_p5 AACTCGTCCAACGGATTAGC TCGAACGAATCACACTTTTACC 50.0 40.9 58.0 57.0 1256-1431 bp 1.0 61.4