Hotspot

ndhE

SSC · 126095-126400 · 306 bp

Back to Gagnebina

Pi 0.00327

MarkerSeek score 39.70

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 1
pi: 0.00327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	CCATTTTGTAGCTGCAGCAC	ACCCATGCTTCGAGTTGTTT	50.0	45.0	58.0	58.0	1410-1411 bp	1.0	80.1
ndhE_p2	CCATTTTGTAGCTGCAGCAC	GCTTCGAGTTGTTTCATGCC	50.0	50.0	58.0	58.0	1404-1405 bp	1.0	80.0
ndhE_p3	TTTCTGCCTTTTCGTTGGGA	ACCCATGCTTCGAGTTGTTT	45.0	45.0	57.9	58.0	1474-1475 bp	1.0	79.7
ndhE_p4	TTTCTGCCTTTTCGTTGGGA	GCTTCGAGTTGTTTCATGCC	45.0	50.0	57.9	58.0	1468-1469 bp	1.0	79.6
ndhE_p5	CCATTTTGTAGCTGCAGCAC	AAGAAATCTGTCGGACAGGC	50.0	50.0	58.0	57.9	1364-1365 bp	1.0	79.4