Hotspot

ndhD-ccsA

SSC · 128529-129085 · 557 bp

Back to Gagnebina

Pi 0.00549

MarkerSeek score 62.90

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 557
variable_sites: 3
pi: 0.00549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	CAGTTGACAAGGTCGAAGCT	AGTGGCATCTATGGGCTTTC	50.0	50.0	58.1	57.6	698-702 bp	1.0	77.7
ndhD-ccsA_p2	CAGTTGACAAGGTCGAAGCT	GTGGCATCTATGGGCTTTCT	50.0	50.0	58.1	57.6	697-701 bp	1.0	77.7
ndhD-ccsA_p3	TCAGTTGACAAGGTCGAAGC	AGTGGCATCTATGGGCTTTC	50.0	50.0	58.1	57.6	699-703 bp	1.0	77.7
ndhD-ccsA_p4	TCAGTTGACAAGGTCGAAGC	GTGGCATCTATGGGCTTTCT	50.0	50.0	58.1	57.6	698-702 bp	1.0	77.7
ndhD-ccsA_p5	CAGTTGACAAGGTCGAAGCT	TGGCATCTATGGGCTTTCTT	50.0	45.0	58.1	56.9	696-700 bp	1.0	71.5