Hotspot

rps3-rps19

LSC · 88710-89120 · 411 bp

Back to Gagnebina

Pi 0.0438

MarkerSeek score 77.70

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 18
pi: 0.0438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	ACTATCAACTTCCGGGGACA	50.0	50.0	57.5	58.1	502-852 bp	1.0	78.1
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	ACTATCAACTTCCGGGGACA	50.0	50.0	58.6	58.1	496-846 bp	1.0	77.2
rps3-rps19_p3	TACCAAGTCTGAAACCGAGC	GGGGACATGCGAAAACTGAT	50.0	50.0	57.5	58.5	489-839 bp	1.0	74.3
rps3-rps19_p4	TGGTTGTGCAAACCAAAGAG	ACTATCAACTTCCGGGGACA	45.0	50.0	57.0	58.1	540-890 bp	1.0	73.9
rps3-rps19_p5	TCTGAAACCGAGCGGATTTA	ACTATCAACTTCCGGGGACA	45.0	50.0	57.0	58.1	495-845 bp	1.0	73.4